Oxymatrine suppresses p-STAT1/PIAS1 signaling in LPS-induced human mesangial cells proliferation
- VernacularTitle:氧化苦参碱对脂多糖诱导的人肾小球系膜细胞增殖p-STAT1/PIAS1信号分子的影响
- Author:
Hongxing DANG
;
Yu JIN
;
Yuning LI
;
Jizu LING
;
Jie SU
- Publication Type:Journal Article
- Keywords:
human mesangial cells;
proliferation;
oxymatrine;
JAK/STAT;
p-STAT1/PIAS1
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of oxymatrine(OM) on the expressions of p-STAT1 and PIAS1 signaling molecules at protein and mRNA levels in the proliferation of the human mesangial cells(HMC) induced by lipopolysaccharide(LPS) and explore the relationship between them.Methods HMCs were primarily cultured from a 4-month-old aborted human fetus(with informed consent and approved by the Ethics Committee of Lanzhou University),and then divided into 3 groups,that is,control group,LPS group(10 ng/ml) and OM group(LPS 10 ng/ml and OM 320 mg/L).After cultured for 12,24 and 48 h respectively,HMC proliferation were analyzed by methyl thiazolyl tetrazolium(MTT) assay and type Ⅳ collagen in the supernatants were detected by ELISA.At the same time points,the cells lysates were collected for the mRNA and protein expressions of p-STAT1 and PIAS1 by real-time quantitative RT-PCR and Western blot analysis.Results The cell proliferation of LPS group was faster and the type Ⅳ collagen protein was increased more than the control group(P
