Comparison of histopathological staining methods for Cryptococcus neoforman
- VernacularTitle:几种显示新型隐球菌的组织病理学染色方法的应用比较
- Author:
Rong XIN
;
Deyu GUO
- Publication Type:Journal Article
- Keywords:
Cryptococcus neoforman;
staining method
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare several histopathological staining methods in demonstrating Cryptococcus neoforman.Methods Hematoxylin-eosin(HE) method,periodic acid-Schiff(PAS) method,Alcian blue method and Grocott-Gomori's methenamine silver method were carried out in the lung and brain tissue samples from 7 autoptical cases identified with Cryptococcus neoforman infection.Results All these methods demonstrated Cryptococcus neoforman infection,HE method showed unclear pink cell body which were difficult to be found.Alcian blue method displayed blue picture,and PAS method showed violet-red body,and Grocott-Gomori's methenamine silver method presented clear dark black picture which was easy to be distinguished from other components.Conclusion Grocott-Gomori's methenamine silver method is the best method in staining Cryptococcus neoforman.
