Effect of cimetidine on clonal expansion of TCR V? subfamily of T cells in cord blood stimulated by K562 cells
- VernacularTitle:甲氰咪呱对K562细胞诱导脐血T细胞TCR V?亚家族克隆性的影响
- Author:
Hongxia TIAN
;
Chen LIN
;
Shaohua CHEN
;
Lijian YANG
;
Zhenyou JIANG
;
Ke GAO
;
Shijuan GAO
;
Yangqiu LI
- Publication Type:Journal Article
- Keywords:
cimetidine;
T cell receptor V? gene;
chronic myelogenous leukemia;
cord blood
- From:Journal of Third Military Medical University
2003;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of cimetidine on the clonal expansion of TCR V? subfamily of T cells in cord blood after the stimulation by K562 cells in vitro.Methods Cimetidine(1?10-5 mol/L) or K562 cells(1?106/ml) or both of them were respectively cultured with mononuclear cells(MNC) isolated from normal human cord blood for 2 weeks.After the induction,specific cytotoxicity of the proliferated T cells were detected with K562 cells as the target cells.The selective usages and clonal expansion of TCR V? subfa-mily of T cells were analyzed by RT-PCR and genescan technique.Results After induction for 2 weeks,the 3 groups showed the increased cell proliferation,in which specific cytotoxicity of T cells induced by both cimetidine and K562 cells against K562 cells was enhanced significantly compared with the other 2 groups(P
