The Experiment Study on Inhibitory Effect of the Extract of Rhizoma Amorphophalli by Petroleum Ether on Mice Vaccinated with H_(22) Tumor
- VernacularTitle:中药蛇六谷石油醚萃取物对小鼠H_(22)肝癌移植瘤抑制作用的实验研究
- Author:
Peifeng CHEN
;
Qiaofeng WU
;
Guibao NI
- Publication Type:Journal Article
- Keywords:
Rhizoma Amorphophalli;
H22 Liver cancer cell;
antitumor ratio;
apoptosis;
Survivin;
Bax
- From:
Journal of Zhejiang Chinese Medical University
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the inhibitory effect of the extract of Rhizoma Amorphophallus,we set up the Kunming mices which were vaccinated with H22 tumor as experimental model,and explore the possibility mechanism of them.[Method]We adopted the mices vaccinated with H22 tumor as experimental model to observe the influence of different extracts on the antitumor ratio,and the cooperating antitumor effect together with 5-Fu.Using flow cytometry to detect the effect of petroleum ether extract on apoptotic cells.Using immunohistochemical method to observe the influence of this extract to the expression of Survivin and Bax(Bcl-2 associated protein x).[Result]The extract of petroleum ether of Rhizoma Amorphophalli had inhibitory effect of the growth on mice vaccinated with H22 tumor,and the possible mechanism was the downregulation of Survivin expression and upregulation of Bax expression to induce apoptosis of tumor cell.
