Determination of the content of sinapine thiocyanate in Qingrehuashiyangyin granules by HPLC
- VernacularTitle:HPLC法测定清热化湿养阴颗粒中芥子碱的含量
- Author:
Guoqing YUAN
;
Guoying NIU
- Publication Type:Journal Article
- Keywords:
Qingrehuashiyangyin granules;
HPLC;
Sinapine thiocyanate;
Determination
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard of sinapine thiocyanate in Qingrehuashiyangyin granules by HPLC.Methods:HPLC was performed on Phenomenex C18 Column(250mm?4.6mm,5?m) with acetonitrile-0.08mol/L KH2PO4(15:85) as mobile phase.Flow rate was 1.0ml/min and detecting wave length was 326nm.Column temperature at 30℃.Results:The good linear relationship was obtained in the range of 0.3976--1.988?g(r=0.9998),and the average recovery was 99.0%.Conclusion:This method is simple,fast and accurate for the quality control of Qingrehuashiyangyin granules.
