Evaluation on fi ngerprint characteristic of Prunella Species with cluster analysis method
- VernacularTitle:夏枯草属药材指纹特征聚类分析评价
- Author:
Liang FENG
;
Yan CHEN
;
Xiaobin JIA
;
Xiaobin TAN
;
Chenglin GAO
;
Guangmin LIU
;
Zhenzhong SHAO
- Publication Type:Journal Article
- Keywords:
Prunella vulgaris L.;
P.asiatica Nakai;
P.hispida Benth.;
HPLC;
Cluster analysis;
Composition-structure theory
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the HPLC fingerprint of Prunella Species from different places and evaluate it by cluster analysis.Methods:The analysis method of HPLC ngerprint was established.Alltima C18(4.6mm?250mm,5?m) was utilized for separation.The mobile phase consisted of methanol(A) and 0.5% glacial acetic acid(B) with a ow-rate of 1.0ml/min.The detection wavelength was 280nm and the injection volume was 20?l.The column temperature was 30℃.The HPLC ngerprints of Prunella Species from three di erent places were determined and analyzed by SPSS.Results:There is a big di erence among ngerprints of Prunella from di erent places.Prunella from Hubei,Anhui,Jiangxi,Guizhou,Guangxi,Sichuan were divided into a class;Henan,Jiangsu,Zhejiang were divided into another class.Conclusion:the eatsblished ngerprint showed characteristics of Prunella Species from di erent places obviously,and the attribute of Prunella Species can be distinguished e ectively by cluster analysis method.
