Relationship between the sensitivity of human colon carcinoma cells to gefitinib and the expressions of Akt and MAPK
- VernacularTitle:结肠癌细胞对吉非替尼的敏感性与Akt、MAPK表达的关系
- Author:
Li YANG
;
Xiaoxing ZHAO
;
Jianjun LI
;
Houjie LIANG
- Publication Type:Journal Article
- Keywords:
gefitinib;
mitogen-activated protein kinases;
proto-oncogene proteins c-akt;
epidermal growth factor,receptor;
colonic neoplasms;
drug screening assay,antitumor
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the sensitivity of colon carcinoma cells to epidermal growth factor receptor(EGFR) inhibitor,gefitinib,and the downstream proteins of EGFR.Methods The expressions of EGFR proteins of 6 colon carcinoma cell lines(Lovo,HCT116,HT29,LS174T,SW480 and SW620) were determined with immunocytochemistry staining.The inhibitory effects of gefitinib on the growth of colon carcinoma cells were assessed by MTT,and the expression levels of Akt and MAPK as well as their phosphorylated forms(p-Akt and p-MAPK) were assessed by Western blotting.Results EGFR protein expressed in all the Lovo,HCT116,HT29,LS174T and SW480 cells,and the highest expression was found in Lovo cells,but not in SW620 cells.Lovo cells showed the highest,HT29 and SW480 cells showed moderate,sensitivity to gefitinib,while the others showed more or less resistance to gefitinib.No significant difference was found between the growth inhibition and IC50 values among the 6 cell lines despite of being treated with fetal bovine serum or EGF.Akt protein existed in all the cell lines without notable difference.Lovo and SW620 cells showed the least of p-Akt expression(P
