Inhibition of total phenolics acid extracted from Oenanthe Javanica on HBV-DNA,cccDNA in cultured cell line 2.2.15
- VernacularTitle:水芹总酚酸对2.2.15细胞HBV DNA、cccDNA的抑制作用
- Author:
Xuanju WANG
;
Zhengming HUANG
;
Xinbo YANG
;
Jianguo SONG
- Publication Type:Journal Article
- Keywords:
OJTPA;
2.2.15 cells;
HBV-DNA;
HBV cccDNA;
cell culture;
Fluorescence-quantitative PCR
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the anti-hepatitis B virus effect of total phenolics acid extracted from Oenanthe Javanica(OJTPA) with in vitro experiments.Methods HePG2 2.2.15 cells were treated with OJTPA,which were diluted into 500 mg?L-1,250 mg?L-1 and 125 mg?L-1 groups.The test set up positive control group(entecavir,3 mg?L-1) and normal control group.The cells of the 4 th,8 th and 4 th day ceasing treatment were collected.The content of HBV-DNA and cccDNA was determined with Quantitative Real-Time PCR method,and the inhibition rates were calculated.Results OJTPA could obviously inhibit HBV-DNA and cccDNA under the dosages used in the experiment,and showed a dose-dependent relationship.The inbhibitory rates reached the peak of 62.3% and 62.7% for HBV-DNA and cccDNA respectively,and a high inhibitory rate was retained within 3 days of OJTPA withdrawal.Conclusion OJTPA has significant inhibition on HBV-DNA and cccDNA and there is no significant rebound phenomenon.
