Purification of L-amino acid oxidase from Naja atra venom and its effects on endothelial cells
- VernacularTitle:眼镜蛇毒L-氨基酸氧化酶分离纯化及对血管内皮细胞的抑制作用
- Author:
Zhou CHEN
;
Jianjun HUANG
;
Ling XUE
;
Yunlu XU
;
Lishan LIN
- Publication Type:Journal Article
- Keywords:
Naja atra venom;
L-amino acid oxidase;
purification;
HUVEC;
endothelial cells/tubule-forming capacity;
Affinity chromatography
- From:
Chinese Pharmacological Bulletin
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To purify L-amino acid oxidase(LAAO) from the venom of Naja atra and study its effect on endothelial cells.Methods The NAV-LAAO was purified by ion-exchange chromatography and affinity chromatography.The MTT assay and Western blot were used to detect the viability and apoptosis of HUVEC.The tubule-forming was used to study the angiogenesis of cells.Results The NAV-LAAO was purified successfully from the venom of Naja atra.The molecular weight of NAV-LAAO was determined to be 58 ku by SDS-PAGE.NAV-LAAO effectively inhibited the growth and tubule-forming of HUVEC,and the 50% inhibitory concentration(IC50) was 21.42 mg?L-1.Compared with control,the levels of caspase-3 and caspase-8 increased in HUVEC treated with NAV-LAAO.Conclusions The NAV-LAAO is purified successfully from Naja atra venom by ion-exchange chromatography and affinity chromatography.The NAV-LAAO inhibits the growth and tubule-forming capacity of HUVEC in a dose-dependent manner.
