Clone and expression of human cytomegalovirus UL144 gene and its effects on dendritic cells
- VernacularTitle:人巨细胞病毒UL144基因克隆表达及其对树突状细胞功能的影响
- Author:
Huaizhou WANG
;
Gang CAI
;
Qian SHEN
- Publication Type:Journal Article
- Keywords:
human cytomegalovirus;
UL144 gene;
herpes virus entry mediator;
dendritic cell;
immune tolerance
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a recombinant adenovirus vector carrying human cytomegalovirus(hCMV) UL144 gene and to explore the biological characteristics of UL144 gene-modified DCs.Methods:The UL144 gene was amplified from hCMV DNA,which was extracted from hCMV-DNA positive serum.The recombinant adenovirus vector carrying hCMV UL144 gene was constructed with AdEasy system and then transfected into HEK293 cells to create recombinant adenovirus Ad-UL144.The expression of inserted gene was identified by RT-PCR.The recombinant adenovirus was then transfected into mice myeloid dendritic cells.The surface proteins of dendritic cells were analyzed by FACS,and cytokines in supernatant were detected by ELISA.T cell proliferation stimulated by gene-modified DC was examined by 3H-TdR uptake assay.Results:The UL144 gene was successfully cloned into the pAdEasy-1 plasmid.The recombinant adenovirus Ad-UL144 was packed in HEK293 cells,with a viral titer of 3?1010 pfu/ml.DCs infected with AdCMV-UL144 had markedly decreased surface expression of CD80,CD86 and I-Ad(P
