Bevacizumab(Avastin) inhibits corneal neovascularization in rabbits
- VernacularTitle:Bevacizumab(Avastin)抑制兔眼角膜新生血管的实验研究
- Author:
Yuqun ZHANG
;
Qing PENG
;
Peiquan ZHAO
- Publication Type:Journal Article
- Keywords:
bevacizumab;
corneal neovascularization;
vascular endothelial growth factor;
cornea;
aqueous humor
- From:
Academic Journal of Second Military Medical University
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of subconjunctival injection of Bevacizumab(Avastin) on corneal neovascularization(CNV) in rabbits.Methods:Forty-eight New Zealand rabbits were randomly divided into three groups:normal control group(group A,3 rabbits),neovascularization group(group B,9 rabbits) and Bevacizumab treatment group(group C,36 rabbits).Group C was further divided into group C1(1 day small dose group),C2(1 day high dose group),C3(14 days small dose group) and C4(14 days high dose group),with 9 rabbits each group.CNV model was made by suture in group B and C.Animals in group C were injected subconjunctivally with 0.1 ml or 0.2 ml Bevacizumab(25 mg/ml) in the left eyes.The growth of CNV was observed every day after operation and the neovascularization areas calculated.Expression of VEGF in the cornea was detected by immunohistochemistry on day 7,14 and 28 after suture.VEGF content in the aqueous humor was determined by ELISA assay.Results:CNV growth in group C1 and C2 was inhibited significantly compared with that in group B on day 7,14 and 28(P
