THE ANTITUMOR MECHANISM OF SERUM AGAINST GROWTH INHIBITION OF DHA TO A549 LUNG CANCER CELLS
- VernacularTitle:血清对DHA抑制肺癌A549细胞生长的影响及DHA的抗癌机制
- Author:
Zuquan ZOU
;
Xiaohong ZHANG
;
Lina ZHANG
;
Wenhua XING
;
Renjie ZHUO
;
Duo LI
- Publication Type:Journal Article
- Keywords:
DHA;
serum;
protein kinase B;
growth inhibition
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of serum on anticancer activity of DHA and its antitumor mechanism. Method The growth inhibition of DHA on lung cancer A549 cells,and the effect of DHA on serum-induced protein kinase B(Akt)activation were assessed by MTT and Western blot respectively. Results DHA markedly induced growth inhibition of A549 cells in low concentration of serum,but promoted growth of A549 cells in high concentration of serum. DHA significantly blocked serum-induced activation of Akt in serum-free medium. Conclusion Serum is obviously against DHA-mediated growth inhibition of A549 cells,and DHA inhibits activation of Akt by lipid peroxidation and blocks growth of A549 cells.
