PCR analysis of clinically isolated Staphylococcus aureus biofilm associated gene
- VernacularTitle:临床分离金黄色葡萄球菌生物膜相关基因的PCR分析
- Author:
Fengjun SUN
;
Qian WANG
;
Peiyuan XIA
- Publication Type:Journal Article
- Keywords:
staphylococcus aureus;
biofilm;
biofilm associated gene
- From:Journal of Third Military Medical University
2003;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of biofilm forming ability with biofilm associated gene of the clinically isolated Staphylococcus aureus for providing basis for the further studies of the mechanisms of biofilm formation.Methods Safranine staining was conducted for the detection of the forming ability of biofilm in 96-well plates.icaAD,icaBC,sar,agr and sigB was amplified by PCR.Results Formation of biofilm could be found macroscopically in 17 out of 27 strains,especially to X387 and X409.icaAD and icaBC were amplified in 22 isolates and sar,agr and sigB in all 27 S.aureus strains.Conclusion ica operon is the key gene for biofilm formation but cooperative action of other genes is needed during the process of biofilm formation.
