Effects of Genistein-magnetic-nanoparticles on the Expression of FAK in Human Hepatocellular Carcinoma Cell line HepG_2
- VernacularTitle:Genistein-磁性纳米微粒对人肝癌细胞中FAK表达的影响
- Author:
Gongping WANG
;
Dongpeng LI
;
Xiubao LIU
;
Yuyuan ZHAO
;
Xiaoshan FENG
- Publication Type:Journal Article
- Keywords:
Genistein;
Hepatocellular carcinoma;
Focal adhesion kinase;
Reverse transcription polymerase chain reaction
- From:
Journal of Medical Research
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Genistein-magnetic-nanoparticles at different concentrations on the growth and expression of focal adhesion kinase(FAK) in human hepatocellular carcinoma cell line HepG2.Methods Activities of HepG2 cells treated by Genistein-magnetic-nanoparticles were examined by MTT assay.The expression of FAK mRNA and protein was respectively detected by immunohistochemistry staining and reversed transcriptase polymerase chain reaction(RT-PCR).Results Genistein-magnetic-nanoparticles at a concentration of 10-80mg/L inhibited the proliferation of HepG2 cells,with obvious concentration-dapendent and time-dependent effect relationships(P
