Generative pathway and mechanism of local angiotensin Ⅱ generation in islets of Syrian golden hamsters
- VernacularTitle:叙利亚金黄地鼠胰岛局部血管紧张素Ⅱ的生成途径与机制探讨
- Author:
Kan SUN
;
Jia SUN
;
Hong CHEN
;
Hua ZHANG
;
Dehong CAI
- Publication Type:Journal Article
- Keywords:
islets of langerhans;
renin-angiotensin system;
chymases
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes in angiotensin Ⅱ(AngⅡ)produced by Langerhan islets of Syrian hamsters after blockage of renin-angiotensin system(RAS)by different inhibitors.Methods Islet cells from Syrian golden hamster were isolated and purified,and angiotensin Ⅰ was added.The experiment was then divided into six groups:control group(PBS was added),captopril group,chymostatin group,aprotinin group,?-antitrypsin group,and captopril+chymostatin group(according to inhibitors added).The content of AngⅡ in supernatant was detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with control group,the AngⅡ content decreased by 42.50% and 50.94% in captopril group and chymostatin group,respectively(P
