Effects of complete Freund's adjuvant-induced thymocyte apoptosis on NOD mice with type 1 diabetes
- VernacularTitle:完全弗氏佐剂诱导胸腺细胞凋亡对NOD鼠1型糖尿病发生的影响
- Author:
Xiaohong NIU
;
Xiaoxi WANG
;
Tiejian JIANG
;
Zhiguang ZHOU
- Publication Type:Journal Article
- Keywords:
freund's adjuvant;
mice,inbred NOD;
diabetes mellitus,type 1;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the preventive effect of complete Freund's adjuvant induced thymocyte apoptosis on NOD mice with type 1 diabetes.Methods Forty-two female NOD mice of 3 weeks old were randomly divided into complete Freund's adjuvant group(CFA)and phosphate buffer saline group(PBS)(21 each).Animals of CFA group received injection of 50?l CFA at hind foot-pad,and those in PBS group received 50?l PBS at the same location.Five mice of each group were sacrificed at the 6th and 12th week,respectively,and the remainders of each group were sacrificed at the 30th week for the examination of insular ? cell apoptosis,thymocytes apoptosis,insulitis severity and diabetes incidence.Results Both the insulitis severity score and the ? cell apoptosis declined(P
