Protective effect of bone marrow mesenchymal stem cells on chemotherapy-induced apoptosis of ovarian granulosa cells
- VernacularTitle:骨髓间充质干细胞对磷酰胺氮芥诱导卵巢颗粒细胞凋亡的保护作用
- Author:
Xiafei FU
;
Yuanli HE
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
granulosa cells;
drug therapy;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-apoptotic effect of bone marrow mesenchymal stem cells(MSCs)on chemotherapy-injured ovarian granulosa cells and its mechanism.Methods Granulosa cells were isolated from female Wistar rats and cultured in vitro in media containing chemotherapeutic agents.The experiment consisted of 4 groups:granulosa cells culture group(control group),co-culture group 1 with MSCs/GCs ratio of 1∶1,co-culture group 2 with MSCs/GCs ratio of 1∶5 and co-culture group 3 with MSCs/GCs ratio of 1∶10.Apoptosis was detected by Annexin Ⅴ.The expressions of Bcl-2 and Bax were assessed using Western blotting.The concentrations of vascular endothelial growth factor(VEGF),hepatocyte growth factor(HGF)and insulin-like growth factor-1(IGF-1)in MSCs media were measured by ELISA.Results The drug could induce apoptosis of granulosa cell,and the apoptotic ratio in control group was 35.04%?5.75%,and in the other three co-culture groups was 12.80%?2.42%,13.94%?3.86%,22.31%?5.67%,respectively.There was significant difference in apoptotic ratio between control group and 3 co-culture groups.The expression of Bcl-2 in co-cultured granulosa cells increased significantly compared with that in control group(P
