Expressions of zinc-finger protein 217(ZNF217)in DDP-sensitive and-resistant human ovarian cancer cell lines
- VernacularTitle:卵巢癌细胞系顺铂化疗敏感株与耐药株中ZNF217的表达
- Author:
Chunrong HE
;
Mei ZHONG
;
Aimin SUN
- Publication Type:Journal Article
- Keywords:
ZNF217 protein,human;
ovarian neoplasms;
cisplatin;
drug resistance,neoplasm
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mRNA and protein expressions of zinc-finger protein 217(ZNF217)in cisplatin(DDP)-sensitive and DDP-resistant human ovarian cancer cell lines.Methods Six strains of ovarian cancer cells,including 3 DDP-sensitive strains(A2780,SKOV-3 and COC1)and 3 DDP-resistant strains(A2780-DDP-R,SKOV-3-DDP-R and COC1-DDP-R)were selected.The relative luminescence unit(RLU)of cells was determined with ATP assay,the IC50 value and resistance index(RI)of DDP-resistant cell lines were calculated.Intracellular localization of ZNF217 protein in the 6 ovarian tumor cell lines was detected by immunofluorescent cytochemistry.The expressions of ZNF217 mRNA and protein were determined by RT-PCR and Western blotting,respectively.Results The IC50 values of DDP to A2780 and A2780-DDP-R were 18.1?2.3mg/L and 47.9?3.8mg/L(P
