Screen of inhibitory effect on ?-glucosidase of 10 kinds of Chinese herbs
- VernacularTitle:10味中药提取物对?-葡萄糖苷酶的抑制作用
- Author:
Shuying HAN
;
Jian LI
;
Chunhua JIANG
;
Jinxiu CHU
;
Yunxia YANG
;
Wei GUO
- Publication Type:Journal Article
- Keywords:
Extraction of Chinese Herb;
?-Glucosidase;
Acarbose(ACAR);
Postprandial blood glucose
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the inhibitory effect on ?-glucosidase of 10 kinds Chinese herbs and to screen the Chinese herbal medicines which have great inhibitory effect on ?-glycosidase.Methods:The ?-glucosidase was extracted from small intestine of rat.The amount of glucose was measured with produced from substrate of malt sugar.The inhibitory effect of 10 kinds of Chinese herbs on ?-glucosidase was observed by this enzyme reaction system.Then disposable gastric perfused malt sugar(2 g/kg) and the extraction screened at the same time,detected the levels of blood glucose after 60 min.The positive control group is acarbose(ACAR) group.Results:The three kinds Chinese herbs(Chishao,Shanzhuyu and Sangbaipi) showed very good inhibitory activities,and they showed obviously concentration-effect curve relationship.Among them,the inhibitary activity of Sangbaipi is stronger than Chishao and Shanzhuyu.While the dose of Sangbaipi reached 10mg/mL,the inhibition rate arrived 80%,which effect was equivalet to the dose of 1mg/ml acarbose.The results of postprandial blood glucose in vitro showed us:Sangbaipi,Chishao and Shanzhuyu can inhibit postprandial blood glucose levels in rats that have been disposable gastric perfused malt sugar after 60min(P0.05).Conclusion:The three kinds of Chinese herbs(Chishao,Shanzhuyu and Sangbaipi) can inhibit ?-glucosidase activity obviously in both vitro and vivo.
