Interleukin-4 decreases expression of epithelial sodium channel ?-subunit in A549 cells
- VernacularTitle:白细胞介素-4对肺泡上皮细胞钠离子通道?亚基调控研究
- Author:
Wei ZHANG
;
Daoxin WANG
- Publication Type:Journal Article
- Keywords:
IL-4;
epithelial sodium channel ?-subunit;
acute lung injury/acute respiratory distress syndrome
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of IL-4 on the expression of epithelial sodium channel(ENaC) ?-subunit.Methods A549 cells were cultured with IL-4 at the concentration of 0,0.1,1 and 10 ng/ml for 12 h or with 1 ng/ml IL-4 for 3,6,12,24 and 48 h.Then ?-ENaC protein and mRNA expressions were detected by Western blotting and RT-PCR respectively.Results IL-4 decreased the expression of ?-ENaC in A549 cells in a time-and dose-dependent manner,via gene transformation.When A549 cells were cultured with IL-4 at the concentration of 1 ng/ml for 3,6,12,24 and 48 h,the expression of ?-ENaC protein and mRNA were decreased in a time-dependent manner.Statistical analysis showed culture for 6 h had a significant difference compared with other time points(P
