Preparation and process optimization of HCPT-loaded PLGA microvesicles by ultrasound
- VernacularTitle:10-羟基喜树碱乳酸-羟基乙酸共聚物超声微泡制备及工艺优化
- Author:
Qing WU
;
Zhaojing ZHU
- Publication Type:Journal Article
- Keywords:
camptothecin;
poly(lactic-co-glycolic acid);
microvesicles;
preparation;
optimization
- From:Journal of Third Military Medical University
2003;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of different technical conditions on the microvesicle size,envelopment rate,morphology of surface and so on in order to select the best technical conditions to prepare HCPT(10-hydroxy-camptothecin)-loaded PLGA microvesicles.Methods Microvesicles were prepared by a water/oil/water emulsion and solvent evaporation method.Various factors related to the envelopment rate and micmparticle size were studied,such as the ratio of water phase and oil phase,ultrasound power,and time,stirring time and so on.Single factor experiments and orthogonal design testing was carried out to optimize the technology of microvesicles preparation.Results The best processing conditions for microvesicles preparation were as following: HCPT 25 mg,PLGA 1.875 g,the ratio of internal and external phases 1∶15,and the PVA concentration 3%.These preparative variables produced global,smooth and glossy microvesicles.Electric charges were probably between-40 to 0 mV,microvesicles sizes ranged from 500 to 1 000 nm,envelopment rate was 79.33%,drug loading to the microvesicles was 0.478 3%,and ultrasonic imaging was clear in vitro.Conclusion Our optimize technical conditions can prepare injectable microvesicles by ultrasound.
