Proteomics research on myocardial tissues of Chinese experimental mini-pig model with coronary heart disease
- VernacularTitle:中国实验小型猪冠心病模型的心肌组织蛋白质组学研究
- Author:
Lan MIAO
;
Jianxun LIU
;
Xinzhi LI
;
Yinghong PAN
- Publication Type:Journal Article
- Keywords:
coronary heart disease;
myocardial tissue;
proteomics;
experimental mini-pig;
two dimensional gel electrophoresis;
MALDI-TOF-MS
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the changes of protein expression pattern of myocardial tissues in mini-pig with coronary heart disease. Methods Based on the model of mini-pig with coronary heart disease,the total proteins of myocardial tissues from each group were separated by two dimensional gel electrophoresis (2-DE).Protein exeprssion pattern in normal and model group was contrasted by Image Master 2D Elite 6.01 analysis software. Then the different proteins were identified by MALDI-TOF-MS. Results Compared with normal group,14 proteins had been determined to change:13 proteins were up-regulated and 1 proteins specifically expressed in mini-pig myocardial tissues of the disease. With the search engine MASCOT,4 proteins including HSPA8 (Hsc70)protein,apolipoprotein A-Ⅳ(apoA-Ⅳ),albumin and desmin were identified.Conclusions These four proteins may play an important role in the occurrence and development of coronary heart disease. The result will be further validated by other biological methods.
