Optimizing the establishment of endometriosis model in rats by orthogonal experiment
- VernacularTitle:正交试验优化大鼠子宫内膜异位症模型
- Author:
Bo CHU
;
Tao JIANG
;
Chunping TANG
;
Chaoyan YANG
;
Mengjuan GONG
- Publication Type:Journal Article
- Keywords:
autotransplantation;
the rat model of endometriosis;
orthogonal experiment
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To probe into the best conditions for the establishment of endometriosis model in rats by the orthogonal experiment method.Methods Autotransplantation was used to establish the endometriosis model in rats by the orthogonal experiment method in which four main factors were considered to optimize the condition of model,according to the volume of heterotopic endometrium.Results The para ligament of uterus was the best place for transplanting.Both dissecting muscular layer and injecting estrin played an important role in the establishment of endometriosis model in rats. The value of four factors decreased progressively according to the injection of estrin,the dissection of muscular layer,the direction of endometrium implantation and oestrus.Conclusions By optimizing the condition for the establishment of endometriosis model in rats by orthogonal experiment,the volume of heterotopic endometrium was improved.A technology platform for pharmacodynamics research was thus offered.
