Inhibition of probucol on the apoptosis of vascular smooth muscle cell induced by hydrogen peroxide
- VernacularTitle:普罗布考抑制过氧化氢诱导血管平滑肌细胞的凋亡
- Author:
Lijuan SHAO
;
Lin SHENG
;
Yajie HU
;
Yanna CHENG
;
Bo JIAO
- Publication Type:Journal Article
- Keywords:
probucol;
vascular smooth muscle cell;
hydrogen dioxide;
apoptosis;
apoptosis signal-regulating kinase-1;
thioredoxin 1(Trx-1)
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of antioxidant probucol on vascular smooth muscle cells(VSMCs) apoptosis induced by H2O2.Methods H2O2 (1 mmol?L-1) was used to induce VSMCs apoptosis.The VSMCs were treated with probucol(100,10,1 ?mol?L-1) for 6 hours.For the evaluation of apoptosis,Annexin V-FITC staining,Hoechest33258 staining and the TUNEL assay were used.The expressions of ASK-1 and Trx-1 were detected by Western blot analysis.Results H2O2 could promote the apoptosis of VSMCs,increase the expression of ASK-1 and decrease the expression of Trx-1.Probucol could attenuate the apoptosis induced by H2O2 in a dose-dependent,down-regulate ASK-1 expression and increase Trx-1 expression.Conclusion Probucol can antagonize the apoptosis of VSMCs induced by H2O2.The mechanism may be correlated with a decreased expression of ASK-1 and an increased expression of Trx-1.
