Effect of aminoguanidine on pulmonary surfactant and alveolar macrophages in the rats induced by LPS
- VernacularTitle:氨基胍对脂多糖诱导的大鼠肺表面活性物质和肺泡巨噬细胞的影响
- Author:
Liping LI
;
Jianxin ZHANG
;
Lanfang LI
- Publication Type:Journal Article
- Keywords:
acute lung injury;
pulmonary surfactant;
aminoguanidine;
alveolar macrophage;
LPS;
rat
- From:
Chinese Pharmacological Bulletin
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of aminoguanidine (AG) on the lipopolysaccharide (LPS)-induced chronological changes of pulmonary surfactant (PS) and alveolar macrophage (AM) of rats.Methods Acute lung injury was induced by injection(iv) of lipopolysaccharide (LPS 5 mg?kg-1). AG(AG group) or saline(control and LPS group) was administrated respectively at 3 h or 6 h after LPS injection for 3 h.The expressions of SP-A mRNA and SP-A protein in the lung tissue were measured by ISH methods and Western blot;the total protein(TP),total phospholipid(TPL) in the bronchoalveolar lavage fluid (BALF) were detected.Rat AM was isolated from the BALF of adult SD rats and harvested by selective plating technique.The concentrations of Tumor necrosis factor-? (TNF-?),Interleukin-6(IL-6),Nitric oxide (NO) and the activity of lactate dehydrogenase (LDH) in the culture supernatants were detected.Results Compared with the controls,SP-A mRNA and SP-A protein in the LPS groups were significantly and progressively decreased. TPL of LPS group in the BALF was significantly decreased whereas TP concentration was significantly increased.Compared with LPS group at the same time points,treatment with AG at 3 h after LPS the expressions of SP-A mRNA and SP-A protein in lung tissue and TPL in BALF were increased markedly,whereas TP was significantly decreased.LPS treated group,LDH activity,the contents of NO,TNF-? and IL-6 in culture medium were significantly increased compared with that of normal group.The LDH activity,NO contents,TNF-? and IL-6 were decreased in AG group compared with that of LPS group.Conclusion Relatively early administration AG(selective inhibitor of iNOS),can protect lung from LPS-induced injury through increasing the expression of SP-A mRNA,SP-A protein and TPL,and inhibiting over-release of a series of cytokines and inflammatory transmitters from AM.
