Effects of Total Saponins of Panax Notoginseng on the Proliferation of Bone Marrow Strowal Cells in Differentiating into Neuron-like Cells in vitro
- VernacularTitle:三七总皂甙对大鼠骨髓基质细胞向神经样细胞增生、分化的影响
- Author:
Dongning LIU
;
Zhenyu ZHANG
;
Xuedong LI
;
Xueli QIU
- Publication Type:Journal Article
- Keywords:
Total saponins of panax notoginseng;
PNS;
Bone marrow stromal cells;
BMSCs;
Proliferation;
Differentiation;
Regulation
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of total saponins of panax notoginseng on the proliferation of bone marrow strowal cells during differentiating into neuron-like Cells in vitro. Methods BMSCs were isolated from femur and tibia of rats and cultured in basal medium(Control),dimethylsulfoxide(DMSO) and butylated hydroxyanisole(BHA) in basal medium as induction group and containing PNS 100mg/L induction medium as PNS group. The morphological changes were observed under inverted microscope.The differentiated BMSCs were detected by immunohistochemistry,and the proliferation were evaluated by MTT assay.Results During induction differentiation,nestin was expressed by all BMSCs with ball-like shape and short process.The nestin expression of PNS group increased as compared with induction group(P
