The Research of Qualitative and Quantitative Method for the Determination of Hesperidin,Magnolol from "He-Wei-Yin" Mixture
- VernacularTitle:“和胃饮合剂”中橙皮苷、厚朴酚的定性定量分析方法研究
- Author:
Nan LI
;
Guozhu HAN
;
Changchuan BAI
- Publication Type:Journal Article
- Keywords:
"He-Wei-Yin" Mixture;
hesperidin;
Magnolol;
Thin layer chromatography(TLC);
High-performance liquid chromatography(HPLC);
Qualitative;
Quantitative
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the determination of Hesperidin,magnolol from "He-Wei-Yin" Mixture,and for its quality control,high-performance liquid chromatography (HPLC) is used.Method After plenty of study about the condition,the final result choosed was as following:an analytical column of HypersiC18(200mm?4.6mm,5?m ) maked by HP was utilized. The column number was 13164421196.The mobile phase was adapted gradient with methanol-1% acetum solution,according to the following profile:0～30min,methanol concentration from 30% to 70%;30～40min,from 70% to 80%;40～50min,from 80% to 30%. The flow rate was 1.0ml/min with the column kept at ambient temperature. All the components had come out in 45 min with the detection wavelength at 294nm. It followed the order of Heperidin,Magnolol according to the retention times in the chromatography.Results In the result,the regression equations were as following:Hesperidin was Y=139.718X+4.511 with a good linearity (r=0.99994). Magnolol was Y=1456.342X+106.506 with a good linearity (r=0.99957) between the peak area and the mass of the standard.The recoveries of the method were as following:in the "He-Wei-Yin" Mixture,for hesperidin,precision of the method RSD=1.87%(n=5),recovery of the method 96.53%(RSD=3.36%,n=3);for Magnolol,precision of the method RSD = 0.34% (n=5); recovery of themethod 93.17%(RSD=4.69%,n=3).At the same condition as above,the determination of content was carried out for six different dose and batch No.Conclusion The method is simple and reliable,easy to operate,suitable for the quality control of Hesperidin and Magnolol in "He-Wei-Yin" Mixture.
