Construction and Identify of Eukaryotic Expression Vector of RNAi hTRET
- VernacularTitle:RNAi人端粒酶反转录酶(hTRET)真核表达载体的构建及鉴定
- Author:
Li FAN
;
Jingwei XU
;
Yu ZOU
- Publication Type:Journal Article
- Keywords:
hTERT;
Eukaryotic expression vector;
siRNA;
DH5a
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct and identify the Eukaryotic expression vector of RNAi hTRET. Methods The hRERT interference sequence was synthesized by artificial way and was directed cloning to the Eukaryotic expression vector pSilencer 3.1 H1 neo,and was identified by pcr,enzyme cutting,sequencing,determining its concentration and purity. Results We construct the Eukaryotic expression vector of RNAi hTRET successfully. Conclusion Construction of Eukaryotic expression vector of RNAi hTRET established foundation for biological effectiveness of telomere and telomerase in cancer cells.
