Influence of Virus Inactivation on Plasma Component and It's Clinical Observation
- VernacularTitle:病毒灭活对血浆成分的影响及临床观察
- Author:
Jianbin LI
- Publication Type:Journal Article
- Keywords:
Methylene blue;
Virus;
Inactivation;
Plasma;
Blood security
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze influence for structure and function of plasma through methylene blue method (MB) in the procedure of virus inactivation.Methods 30 fresh plasma,which were adopted through 400 ml bloods in 6 hours,were choosed randomly and connected with sterile filter with MB. The density of MB was approximately 0.9 ~ 1.3 mol/L. Blood plasma with added MB was placed into the shelf of 4 ℃ virus inactivation and shakem frequently 60 meta /min. Temperature was kept in 4 ℃ within 35min.MB and residual leukocytes were filtered by apparatus of virus inactivation. The surplus of 10 ml blood plasma was immediately placed in -80℃ refrigerator. Concentration of MB,FⅧ:C,FⅤ:C,VWF,Fib of plasma samples was tested before and after virus was inactivated.Results Recovery rates of the volume of plasma,FⅧ:C,FⅤ:C,VWF,Fib respectively were(97.29?2.03)%,(81.43?11.15)%,(80.12?14.03)%,(92.48?8.35)% and (82.86?19.13)% after virus was inactivated through methylene blue method.Conclusion Quality of blood plasma through inactivation of virus with methylene blue method was consistent with national standard and could satisfy clinical need in the precondition of blood safety.There was less influence for blood plasma component.
