Application of MALDI-TOF MS in Renal Ischemia/Reperfusion(I/R) Injury Research
- VernacularTitle:飞行时间质谱技术在肾脏缺血/再灌注(I/R)损伤研究中的应用
- Author:
Xiaoshuang ZHOU
;
Rongshan LI
;
Yufeng QIAO
- Publication Type:Journal Article
- Keywords:
MALDI-TOF MS;
Renal;
Ischemia/ reperfusion (I/R) injury;
Proteomics;
Fibrinogen;
Opypeptide
- From:
Journal of Medical Research
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Proteomics changes from the proteserum which isolated from the rat model of renal ischemia/reperfusion(I/R) injury are detected and investigated by the matrix-assisted UV laser desorption ionization time of flight mass sperctra (MALDI-TOF MS). Methods After the establishment of rat renal ischemia-reperfusion model,the serum samples which we selected respectively in 6,12,24 hours after reperfusion in each group were detected by MALDI-TOF MS analysis. And the peptide fingerprint which existed differences in each group were analyzed to identify.SPSS13.0 software was used to the analysis the data. At the same time,we used Mascot Search to determine their nature in protein database. Results ①the serum which was analyzed by IMAC-Cu bead was detected and had statistically significant peptide fingerprint in the m/z 2481 Da.②the results obtained from peptide mass fingerprint (PMF) were analyzed by Mascot search program for protein identification. We identified it as rat fibrinogen fragment.Conclusion Fibrinogen in kidney ischemia/reperfusion (I/R) injury plays an important role.
