Effect of ChangTong oral liquid on the cell cycle of cultured fibroblasts from normal peritoneum and adhesion
- VernacularTitle:常通口服液对大鼠正常及粘连腹膜成纤维细胞周期的影响
- Author:
Chunxia WANG
;
Xuxin ZENG
;
Qilu HUO
;
Lianbing HOU
- Publication Type:Journal Article
- Keywords:
CTOL;
Fibroblast;
Cell cycle;
Flow cytometry
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of ChangTong oral liquid(CTOL) on cell cycle of cultured fibroblasts from normal peritoneum(NF) and adhesion(AF).Methods:15 SD male rats were randomly divided into 3 groups:normal serum group,2 CTOL groups(medium and high doses).After administration of medication at the different dosages for 7 days,serums were obtained from the abdominal arteries;the prepared tissues were cultured until outgrowth of fibroblasts.In our study,the passage 3-8 cells were used in order to maintain comparability;The fibroblasts in desired condition were plated in 10% FBS,then divided into three groups:rat serum normal group,CTOL groups(medium and high dose).Cells were cultured respectively for 12,24 and 48 h,and analyzed by flow cytometer.Results:Compared with serum normal group of NF,CTOL medium dosage could increase the proportion of NF in the G1 stage at 12,24 and 48h,but only CTOL high dosage had significance(P
