HPLC quantitative analysis and fingerprint study of the chief flavonoids in scutellaria baicalensis
- VernacularTitle:黄芩药材中主要黄酮类成分含量测定及指纹图谱研究
- Author:
Yongyan ZHOU
;
Xiumin CHENG
;
Haiying YU
;
Ying WANG
- Publication Type:Journal Article
- Keywords:
Baicalin;
Baicalein;
Wogonin;
Assay;
HPLC fingerprint
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To set up the HPLC fingerprints of Scutellaria baicalensis collected from different regions and develop a HPLC method for the quantitative analysis of the chief Flavonoids in Scutellaria baicalensis.Methods:The HPLC separation was performed on SHIMADZU VP-ODS column(250mm?4.6mm,5?m),gradient eluted using 0.1% citric acid-acetonitrile solvent system and detected at 276nm and 200nm to 400nm with a DAD detector.The flow rate was 1.0ml/min.The fingerprints of different samples were compared with similarity evaluation software.Results The HPLC fingerprints of Scutellaria baicalensis was set up,in the fingerprints,17common peaks of flavonoids were confi rmed.But the contents of the chief Flavonoids had a big difference,in which the baicalin had the biggest content(18.32%) in cottage Scutellaria baicalensis of Yiyuan County;the baicalein and wogonin had the biggest content(2.31%&0.73%) in wild Scutellaria baicalensis of Northeastern China.Conclusion:This method showed high precision,good repeatability,stability and all of the content were separated well,so it can be useful as the means to assess the quality of Scutellaria baicalensis.
