Studies on the mechanism of antisense telomerase reverse transcriptase in inhibiting the proliferation of pulmonary artery smooth muscle cells induced by 5-hydroxytryptamine
- VernacularTitle:反义端粒酶反转录酶对5-羟色胺诱导的肺动脉平滑肌细胞增殖抑制的机制研究
- Author:
Jingchun SONG
;
Guoming HUANG
;
Xiaowen TU
;
Zhongru DING
;
Huaiyu QIAO
- Publication Type:Journal Article
- Keywords:
telomerase;
oligorinucleotides, antisense;
serotonin
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of antisense telomerase reverse transcriptase (TERT) on 5-hydroxytryptamine (5-HT) induced proliferation and cell cycle of pulmonary artery smooth muscle cells (PASMCs). Methods PASMCs were cultured and divided into four groups: control group (cultured in RPMI-1640 culture medium), 5-HT group (cultured in culture medium containing 5-HT), antisense oligonucleotide (ASODN) group (cultured in culture medium containing 5-HT and ASODN TERT), and sense oligonucleotide (SODN) group (cultured in culture medium containing 5-HT and SODN TERT). The apoptosis of PASMC was observed by fluorescence microscopy with Hoechst staining. Apoptosis and cell cycle was analyzed with flow cytometry. Expression of proliferated cell nuclear antigen (PCNA) was determined by immunohistochemistry staining. Results Hoechst staining showed that apoptosis in 5-HT group (161?33) was significantly higher than that in control group (63?16, P
