Lentiviral vector for RNA interference of human Snail gene inhibits proliferation and invasion of nasopharyngeal carcinoma 5-8F cell line
- VernacularTitle:人Snail基因RNAi慢病毒载体对鼻咽癌5-8F细胞增殖和侵袭的影响
- Author:
Li CHEN
;
Qiuzhen LIU
;
Jihua QIU
;
Feng JIAO
;
Kaitai YAO
- Publication Type:Journal Article
- Keywords:
Snail;
RNA interference;
nasopharyngeal carcinoma;
proliferation;
invasion
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a recombinant lentiviral expression vector for RNA interference (RNAi) of human Snail gene and to study its effects on the proliferation and invasion of nasopharyngeal carcinoma cell line 5-8F. Methods The effective sequence of short hairpin RNAs (shRNA) targeting Snail gene was designed and cloned into the linear pLVTHM vector after enzyme digestion. After confirmation by DNA sequencing, 5-8F cells were infected with the viral supernatants. The cells with stable Snail gene knock-down were separated by fluorescence activated cell sorter (FASC). The expression of Snail mRNA was detected by real time RT-PCR. MTT and cell invasion assay were used to detect the proliferation and invasion of 5-8F cells after plVTHM-siSnail transfection. Results The lentivirus vector plVTHM-siSnail was constructed successfully. The separated 5-8F-plVTHM-siSnail exhibited significant knock-down of Snail mRNA expression. Slower proliferation and decreased cells to permeate through the Matrigel were found after plVTHM-siSnail transfection (P
