Study on piezoelectric quartz crystal microarray immunosensor for the detection of Agkistrodon acutus venom
- VernacularTitle:压电免疫传感器阵列检测尖吻蝮蛇毒的初步研究
- Author:
Minghua LIU
;
Jie YAO
;
Qiang XIANG
;
Bo ZHANG
;
Weiling FU
- Publication Type:Journal Article
- Keywords:
piezoelectric;
immunosensor;
array;
venom;
Agkistrodon acutus
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possibility of piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom. Methods Microarray immunosensor with quartz crystal of 10 MHz AT-cut and 2?5 gold-coated electrodes was prepared. The thiol-treated venom antibody was immobilized by a self assembling device for the detection of the standard fluid for different concentrations of the venom. Results Experimental results showed that the optimal concentration of the antibody was 3.0 g/ml with the response time of 40 minutes. The piezoelectric immunosensor could well respond to homologous venoms. Within the range of 0.1~4.0 g/ml, the frequency shifts were linearly dependent on the venom concentration. Conclusion Piezoelectric microarray immunosensor for the detection of Agkistrodon acutus venom is of high specificity of response, high sensitivity, and simple operation without marking. The technique of piezoelectric microarray immunosensor is possible to test snakebite quickly, quantitatively, and instrumentally.
