Effect of CYP1A1 on intracellular Ca~(2+) in human vein endothelial cells
- VernacularTitle:CYP1A1对人血管内皮细胞内钙的影响
- Author:
Xuan YE
;
Yuguang WANG
;
Zengchun MA
;
Yue GAO
- Publication Type:Journal Article
- Keywords:
cytochrome 1A1;
arachidonic acid;
intracellular Ca~(2+)
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To evaluate the effect of CYP1A1 on intracellular Ca2+ in human vascular endothelial cells(HVEC304).Methods The expression plasmid of human CYP1A1 was constructed,HVEC304 was transfected,and the intracellular calcium ion level was monitored by microfluorescent technique.Result The expression plasmid of human CYP1A1 was constructed and its expression location was extranuclear.In HVEC304 cells over-expressing CYP1A1(HVEC304-CYP1A1),Ca2+ basal level decreased obviously(P
