The differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide
- VernacularTitle:二烯丙基三硫诱导人胃癌细胞凋亡相关基因的差异表达
- Author:
Yali TAN
;
Hao JIANG
;
Wenxiang DAI
;
Xiaoping WU
;
Zhangwen TANG
;
Qi SU
- Publication Type:Journal Article
- Keywords:
diallyl trisulfide;
gastric cancer;
apoptosis;
gene array
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide(DATS).Methods Growth inhibition against MGC803 cells was assayed by MTT assay;The apoptosis induced by DATS was assessed by Flow cytometry and fluorescent microscope.The apoptosis-associated gene expression of MGC803 cell treated with DATS was determided by Human Apoptosis Gene Array.Apaf-1 and SODD genes were confirmed by RT-PCR.Results DATS had significant growth inhibitory activity against MGC803 cells,inhibition ratio increased from 11% to 78% at 4,8,12,16 and 24 mg?L-1 for 72 h(P
