The effect of kanamycin on the change of calcium concentration in outer hair cells caused by acetylcholine
- VernacularTitle:卡那霉素对乙酰胆碱引起外毛细胞内钙离子浓度变化的影响
- Author:
Qinghua GAO
;
Yu YANG
;
Shuice LIU
;
Hao TANG
- Publication Type:Journal Article
- Keywords:
outer hair cells;
Ca~(2+);
kanamycin;
acetylcholine
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effect of kanamycin on the change of Ca2+ concentration in outer hair cells caused by acetylcholine to explore the relationship between kanamycin ototoxicity and the cochlear efferent nervous system.Methods After the guinea pigs were killed by decapitation,the OHCs were isolated in artificial perilymph and divided into three groups:control group,acetylcholine(ACh) group and kanamycin + ACh group.OHCs were loaded with Fluo-3/AM for 30 min and then the change of Ca2+ fluorescence intensity was observed.Results Ca2+ fluorescent intensity in OHCs remained basically unchanged in control group,increased 3.06 times(n=6) in ACh Group,and increased 1.75 times(n=6) in kanamycin +ACh Group.Conclusions Kanamycin can obviously inhibit the increasing of Ca2+ concentration in OHCs caused by ACh.
