Effects of nanoscale titanium dioxide on intercellular gap junction communication in human lung fibroblasts
- VernacularTitle:纳米二氧化钛颗粒对人肺成纤维细胞缝隙连接通讯的影响
- Author:
Kun QI
;
Furong DENG
;
Xinbiao GUO
- Publication Type:Journal Article
- Keywords:
Titanium dioxide;
Nanotechnology;
Fibroblasts;
Gap junctions
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of nanoscale titanium dioxide(Nano-TiO2) on gap junctional intercellular communication(GJIC) between human lung fibroblasts.Methods: Human lung fibroblasts were exposed to two different sizes Nano-TiO2(25 nm and 80 nm) for 24 hours at the concentrations of 0,10,20,40 and 80 mg/L,respectively.The GJIC between cells was measured by using fluorescence redistribution after photobleaching(FRAP) assay.Results: The results showed that GJIC between cells was significantly inhibited both by 25 nm and 80 nm Nano-TiO2 in a dose-dependent manner.The fluorescence recovery rate was 20.81%?1.93% in control group,7.26%?0.91%(P
