PCR - based random amplified polymorphic DNA fingerprinting of vibrio cholerae 01 in Vietnam
- Author:
Nguyen Binh Minh
- Publication Type:Journal Article
- Keywords:
DNA
- MeSH:
DNA;
Random Amplified Polymorphic DNA Technique;
Vibrio cholerae;
DNA Fingerprinting
- From:Journal of Preventive Medicine
2003;13(5):27-30
- CountryViet Nam
- Language:Vietnamese
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Abstract:
The random amplified polymorphic DNA (RAPD) technique was used to distinguish 60 strains of Vibrio cholerae 01 E1 Tor isolated from patients in 1991-2002 period. There were 6 strains isolated from HoChiMinh city, 43 strains from Nha Trang, 8 strains from Hanoi and 3 strains from Tay Nguyen Highland. In RAPD technique, DNA oligomers with 10 nucleotids was selected randomly using to synthese prime DNA from the sites of properly paired aminoacids. Strain specific amplified DNA was created. AP42 and AP46 were used to identify the gene of various strains in diverse areas. Results of RAPD showed that these strains of bacteria had a same origin. RAPD technique can be used to supervise the transmission of bacteria in human
