Effects of uric acid on TGF-?1 and Smad3 expression in human urethral fibroblasts
- VernacularTitle:尿酸对人尿道成纤维细胞TGF-?1、Smad3的影响
- Author:
Xu GUO
;
Gensheng LU
- Publication Type:Journal Article
- Keywords:
urethral scar;
fibroblast;
TGF-?1;
urine;
uric acid
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of urine on TGF-?1 and Smad3 expression in cultured human urethral fibroblasts and explore the mechanism of urethral scar fibrosis.Methods The human urethral fibroblasts were cultured and stimulated by urine or 350 ?mol/L uric acid.The mRNA and protein expression of TGF-?1 and Smad3 were measured by PT-PCR and immunofluorescent staining,respectively.Collagen Ⅰlevels in the supernatants in different groups were detected by ELISA.Results TGF-?1 and Smad3 were detected in human urethral fibroblasts by immunofluorescent staining.After being presented with urine or uric acid at physiological concentration,both TGF-?1 and Smad3 in human urethral fibroblasts were increased significantly at both the protein and mRNA levels(P
