Experimental Study Inhibition Proliferation and Mechanism of Rosiglitazone on HL-60 Cell
- VernacularTitle:罗格列酮抗人HL-60细胞增生作用及其作用机制
- Author:
Wenxiang DAI
;
Li WANG
;
Yanhua CHEN
;
Jianguo CAO
- Publication Type:Journal Article
- Keywords:
Rosiglitazone;
HL-60 cell;
Proliferation;
Apoptosis
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of rosiglitazone on human leukemia cell line HL-60 in vitro.Methods Inhibition of HL-60 cell proliferation was shown by MTT array;cell cycle and apoptosis of human leukemia cell line HL-60 was detected by flowcytometry(FCM);expression of PPAR?,p21WAF1,Bax and bcl-2 was examined by SP immnocytochemical and western blotting.Results Rosiglitazone significantly inhibited proliferation of HL-60 cell and the inhibition effects had a dose-and time-dependence.The inhibition fraction of HL-60 cell to 100?mol/L rosiglitazone for 48h was 77%.Flow cytometry analysis revealed that proliferation of HL-60 cell treated with 10~100?mol/L rosiglitazone significantly inhibited.50~100?mol/L Rosiglitazone cold induce apoptosis of HL-60 cell.SP immnocytochemical found the nuclear translocation on PPAR?,and western blotting revealed that the Bax expression was increased,while the bcl-2 and NF-?B expression was decreased.The expression of PPAR?,Bax,bcl-2 and NF-?B had no change by GW9662,a specific inhibitor of PPAR?.Conclusion Rosiglitazone could induce apoptosis in HL-60 cells which may be involved in the activation of PPAR? and down-regulation of Bax and up-regulation of Bcl-2 and NF-?B protein.
