A Study of the Reverse Effect of Tetramethylpyrazine on the Resistant Cell Strain A2780/ADM of Human Ovarian Carcinoma
- VernacularTitle:川芎嗪对人卵巢癌耐药细胞株A2780/ADM逆转作用实验研究
- Author:
Xulin ZHAO
;
Guochang XU
- Publication Type:Journal Article
- Keywords:
TMP adriamycin;
A2780/ADM cell line;
Ovarian carcinoma
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the reverse roles of reverse effect of tetramethylpyrazine on the resistance to adriamycin in human ovarian carcinoma cell line A2780/ADM.Methods The drug sensitivity of A2780/ADM cells was measured by MTT assay.The intracellular concentration of ADM was determined by fluorescent spectrophotometry.The changes of cell apoptosis percentage of A2780/ADM was demonstrated by flow cytometry.Results The IC50 value of TMP in A2780/ADM cell line was significantly decreased by the non-cytotoxic dose or low-cytotoxic dose of TMP.The resistance reversing effect comparing with the IC50 value of ADM cells was respectively 2.07 folds.The intracellular ADM concentration in A2780/ADM cells was significantly elevated by TMP at the same dose and the cell apoptosis percentage was significantly increased by TMP.The expression of P-gp was reduced significantly by TMP.Conclusion TMP could reverse the resistance of human ovarian carcinoma of A2780/ADM cell line to ADM,which may be related to the increased intracellular.
