Effects of Tissue Inhibitor-3 of Matrix Metalloproteinases Gene-Transfected Vascular Smooth Muscle cells Transplantation on Initial Myocardium Function After Acute Myocardial Infarction
- VernacularTitle:TIMP-3基因转染血管平滑肌细胞移植对大鼠急性心肌梗死后心功能的影响
- Author:
Guangwei LI
;
Hongzhi MIAO
;
Zhibo JIA
- Publication Type:Journal Article
- Keywords:
Tissue inhibitor-3 of matrix metalloproteinases gene;
Acute myocardial infarction;
Myocardium remodeling;
Gene therapy
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of tissue inhibitor-3 of matrix metalloproteinases(TIMP-3)Gene-transfected vascular smooth muscle cells(VSMCs)transplantation on heart function after acute myocardial infarction(AMI)in rats and to explore the potential mechanisms.Methods Wistar rats were produced AMI models by ligating the descending left coronary artery.Rats were survived and divided into 3 groups randomly(n=18):0.5mlDMEM containing 1?106 TIMP-3 gene-transfected VSMCs(group A),1?106 VSMCs(group B)or 0.5 ml DMEM without cell(group C)were injected into the ischemic myocardium immediately.Ischemic myocardium samples were harvested at 3 day after operation.The heart function was observed through the tissue functional examination.The activity of TIMP-3 gene-transfected VSMCs were measured by immunohistochemical method.mRNA of TIMP-3 and matrix metalloproteinase 9(MMP-9)were determined by RT-PCR.Results VSMCs were cultivated and had a high purity(98%).TIMP-3 gene was transfected into VSMCs successfully.Three day after operation in group A the average percentage of LVIDd、LVIDs、EDV and ESV were significantly higher than group normal(P
