Antitumor effect and its mechanism of actinidia Chinensis planch polysaccharide on B16-bearing mice
- VernacularTitle:中华猕猴桃多糖对小鼠皮下移植瘤B16抑制的机制研究
- Author:
Senlin SHI
;
Guofeng PAN
;
Xiaodong ZHANG
;
Changyu LI
;
Weihong GE
- Publication Type:Journal Article
- Keywords:
ACPS;
Antitumor;
Mechanism;
Immunization;
Apoptosis
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antitumor effect of Actinidia Chinensis Planch polysaccharide(ACPS)in B16-bearing C57BL/6 mice and approach its mechanism.Methods:The C57BL/6 mice model was established by B16 subcutaneous inoculation,giving the polysaccharides from vena caudalis injection,and flow cytometer(FCM)was used to detect the distribution of tumor-cell cycle,and electron microscope was used to survey morphologic transformation about apoptosis.Results:ACPS can inhibit the growth of B16,the high,middle dose groups obviously restrained the tumor with the rate of 48.67%,40.90%and the control group 24.13%,and classical apoptosis corpuscles had been found through electron microscope in ACPS groups.Compared with control group,the ACPS promoted the spleen-index of B16-bearing mice and cut down the proliferation index,and the G1/S phase was at growth-inhibitory concentrations judged by the distributing analysis on cell-cycle.Conclusion:ACPS had obvious effect of restraining B16 and promoting the spleen index of tumor-bearing mice,which maybe due to its function of regulating immunization and the distributing of cell-cycle.
