Effect of high glucose on expression of human brain microvascular endothelial cellular adhesion molecule and formation of endogenetic toxins
- VernacularTitle:高糖对人脑微血管内皮细胞黏附分子表达的影响及在内毒形成中的作用
- Author:
Yaqiong LIU
;
Lingqun ZHU
;
Shuoren WANG
;
Yunling ZHANG
;
Hong ZHENG
- Publication Type:Journal Article
- Keywords:
High glucose;
Human brain micro-vascular endothelial cell;
Adhesion molecule;
Endogenetic toxins
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of high glucose on formation of endogenetic toxins and expression of intercellular cell adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) of human brain microvascular endothelial cells.Methods:Cultured HBMEC was used as target cells,anhydrous glucose was added to the cell medium to prepare the injury model of human endothelial cell.Methyl thiazolyl tetrazolium method was used to examine the cell activity in a high glucose environment.Immunocytochemistry method was used to detect the expression of ICAM-1 and VCAM-1 of HBMEC.Results:The expressions of ICAM-1 and VCAM-1of HBMEC were enhanced by high glucose after pretreating HBMEC for 24,48 and 72h(P
