Establishment of rat model of syndrome of stagnation of liver qi and primary study using metabonomics
- VernacularTitle:“肝郁证”大鼠模型的建立及代谢组学的初步研究
- Author:
Shu XU
;
Hebing CHEN
;
Hong LI
;
Qi ZHANG
;
Hongbing CAI
;
Xianzhong YAN
;
Zhiping LV
- Publication Type:Journal Article
- Keywords:
Syndrome of stagnation of liver qi;
Metabonomics;
1H NMR;
Rat models;
Syndrome of TCM
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish and validate the rat model of syndrome of stagnation of liver qi,followed by a primary study on this model with 1H NMR based on metabonomics to explore the essence of syndrome of stagnation of liver qi. Methods:Twelve Wistar male rats were randomly divided into two groups(model group and control group).The rats of model group were restrained by special equipment for 21 days to get into stagnation of liver qi.The behavior,fluid consumption test and plasma CORT of rats were recorded.At 22th day,animals were sacrificed and biopsies of gastric mucosa and adrenal gland were collected for pathological check,and serum samples for 1H NMR spectroscopy.The NMR data were analyzed using principal component analysis.Results:There were abnormal behaviors,such as decrease of elusion,slackness,looser stools,and matte fur were observed among model group rats.After one week the body weight of model group was significantly lower than that of control group(P
