In vitro propagation of Polyscias fruticosa (L.) Harms by direct regeneration from shoot tips
- Author:
Vu Hoai Sam
- Publication Type:Journal Article
- Keywords:
Polyscias fruticosa (L.) Harms
- MeSH:
Araliaceae;
Regeneration
- From:Journal of Medicinal Materials - Hanoi
2005;10(5):135-137
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Study on preserved propagation of Polyscias fruticosa (L.) Harms – Araliaceae by direct regeneration from 3-years-old shoot tips. Shoot tips of 1.0 to 1.5cm long taken from field growing Polyscias fruticosa (L.) Harms were sterilized and cultured in Murashige and Skoog (MS) medium supplemented with 1.0mg/l BAP and 0.5mg/l IBA. After 8 weeks, these shoots began to regenerate directly from axil but quantity was not numerous and gave 2-3 new shoots after 4 more weeks. The results of rapid propagation of Polyscias fruticosa (L.) Harms’s shoots after 1, 3 and 4 months culture in MS + 2.0mg/l BAP + 0.5mg/l IBA environment showed that it was the best combination for shoot proliferation. The single shoots gained 2-3cm long were separated from shoot bushes and cultured in MS + 1.0mg/l IBA + 0.5mg/l BAP environment to make root the best. In this environment, shoots began to rooting after 11 days, gained 100% of rooting after 20 days. The plantlets were successfully transferred to PE bags. After 2 weeks, plantlets grew new roots with the rate of live 80-95%
