Effects of pulsed electromagnetic fields on BMD and TGF-?_1 in disuse osteoporosis rats
- VernacularTitle:脉冲电磁场对废用性骨质疏松大鼠骨密度和血清转化生长因子?_1的影响
- Author:
Weiwei SHEN
;
Jianhua ZHAO
- Publication Type:Journal Article
- Keywords:
pulsed electromagnetic fields;
disuse osteoporosis;
bone mineral density;
transforming growth factor-beta 1
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pulsed electromagnetic fields(PEMF)on the bone mineral density(BMD)and serum level of transforming growth factor-beta 1(TGF-?1)in the disuse osteoporosis(DOP)rats.Methods Eighty 4-month-old female SD rats were randomly divided into 4 matched groups according to their weight:control group(INT group),DOP group,calcitonin group(CT group)and PEMF group.Rats in INT group were raised with the average amount of food consumed by the DOP rats.Right hindlimbs of rats in DOP,CT and PEMF groups were immobilized by tibia-tail fixation to establish DOP model as Zarzhevsky described.Rats in CT group were injected with calcitonin(2 IU/kg,i.p,once a day),and rats in group PEMF were irradiated with PEMF(8 Gs,15 Hz,a pulse time of 8 ms)immediately after operation.The rats(5 animals per time of each group)were killed at 1,2,4 and 8 weeks after operation and their right hindlimbs were resected.The BMD and serum TGF-?1 concentration were measured with a dual energy X-ray bone densitometer and ELISA respectively.Results Significant difference was observed in the femur proximal BMD between rats with right hindlimb immobilization(DOP group)and INT group 2 weeks later(P
