Effect of stromal cells derived from different origins on proliferation and apoptosis of KM3 cells
- VernacularTitle:不同来源基质细胞对骨髓瘤KM3细胞增殖和凋亡的影响
- Author:
Li GAO
;
Xi ZHANG
;
Lei GAO
;
Xinghua CHEN
- Publication Type:Journal Article
- Keywords:
multiple myeloma;
human umbilical cord blood-derived stromal cells;
bone marrow stromal cells;
proliferation;
apoptosis
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of the human umbilical cord blood-derived stromal cells(hUCBDSCs)and multiple myeloma bone marrow stromal cells(MM BMSCs)on the biological characteristics of KM3 cells.Methods After KM3 cells were co-cultured with hUCBDSCs or MM BMSCs,the proliferation,cell cycle and the apoptotic rate were determined by growth curve plotting and flow cytometry respectively.Real-time Q-PCR was used to measure the mRNA expressions of Bcl-2 and Bax.Results As compared with MM BMSCs,hUCBDSCs markedly suppressed the proliferation of the KM3 cells.KM3 cells in the KM3/MM-BMSCs co-culture group were arrested in G0/G1 phrase,while KM3 cells in the KM3/hUCBDSCs co-culture group predominantly in S phrase.The apoptotic rate of KM3 cells in the KM3/hUCBDSC co-culture group were higher than that in the KM3/MM-BMSCs co-culture group.Conclusion In comparison with MM BMSCs,hUCBDSCs inhibit the proliferation of KM3 cells and promote the apoptosis.
